rabbit anti human il 1β polyclonal antibody  (Proteintech)

Journal: Cell reports

Article Title: CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout

doi: 10.1016/j.celrep.2023.112265

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Membranes were blocked (5% non-fat dry milk, 0.1M Tris-buffered saline, pH 7.4, 0.1 % Tween 20) for 1h at room temperature followed by incubation with primary antibodies for 12-16h at 4°C as indicated with rabbit monoclonal anti-human caspase-1 (Cell Signaling Technology, D7F10), rabbit monoclonal anti-cleaved human caspase-1 (Cell Signaling Technology, D57A2), mouse monoclonal anti-mouse caspase-1 (p20) (AdipoGen, Casper-1, AG-20B-0042), mouse monoclonal anti-mouse caspase-1 (p10) (AdipoGen, Casper-2, AG-20B-0044-C100), rabbit monoclonal anti-mouse gasderminD (Abcam, Ab209845), rabbit monoclonal anti-cleaved human gasdermin D (Cell Signaling Technology, E7H9G), rabbit monoclonal anti-gasdermin D (Cell Signaling Technology, L60), rabbit monoclonal anti-cleaved mouse gasdermin D (Abcam, Ab209845), mouse monoclonal anti-NLRP3 (Adipogen, Cryo-2), rabbit polyclonal anti-NLRP3 (Cell Signaling Technology, D4D8T), mouse monoclonal anti-GFP (Santa Cruz Biotechnology, B-2, SC-9996), rabbit monoclonal anti-GFP (Cell Signaling Technology, D5.1, 2956S), alpaca single-domain GFP Selector beads (NanoTag Biotechnologies, N0310), rabbit polyclonal anti-ASC (AdipoGen, AL177; AG-25B-0006-C100), mouse monoclonal anti-β-tubulin (DSHB, AA12.1), rabbit polyclonal anti-human total IL-1β (Santa Cruz Biotechnology, SC7884), rabbit monoclonal anti-human cleaved IL-1β (Cell Signaling Technology, D3A3Z), rabbit monoclonal anti-mouse cleaved IL-1β (Cell Signaling Technology, 52718S), mouse monoclonal anti-mouse mature IL-1β (Cell Signaling Technology, 12242S), rabbit monoclonal anti-cleaved PARP (Cell Signaling Technology, #5625), rabbit polyclonal anti-caspase-9 (Cell Signaling Technology, #9504), rabbit polyclonal anti-caspase-8 (Cell Signaling Technology, #4927). rabbit monoclonal anti-caspase-3 (Cell Signaling Technology, #14220) and rabbit polyclonal cleaved anti-caspase-3 (Cell Signaling Technology, #9661).

Techniques: Purification, Recombinant, Protease Inhibitor, Staining, Magnetic Beads, Enzyme-linked Immunosorbent Assay, TaqMan Assay, LDH Cytotoxicity Assay, CyQUANT Assay, cDNA Synthesis, Proximity Ligation Assay, Western Blot, Software

Journal: Annals of Translational Medicine

Article Title: NLRP3 mediates trophoblastic inflammasome activation and protects against Listeria monocytogenes infection during pregnancy

doi: 10.21037/atm-22-4120

Figure Lengend Snippet: Listeria monocytogenes triggers caspase-1 activation and IL-1β secretion in mouse placentas. (A,C) Western blot analysis of caspase-1, IL-1β, NLRP3, and ASC in mouse placentas. β-tubulin served as a loading control. (B) IL-1β ELISA in the mouse placentas (n=18 placentas). The data are expressed as the means ± SE. *, P<0.05; ***, P<0.001. IL, interleukin; ASC, apoptosis-associated speck-like protein containing a caspase recruitment domain; SE, standard error. n.s., not statistically significant.

Article Snippet: Rabbit anti-human cleaved IL-1β polyclonal antibody (#2021) was obtained from Cell Signaling Technology (CST, Shanghai, China). β-Tubulin monoclonal antibody (Tianjin Sungene Biotech, China) was used as an internal control.

Techniques: Activation Assay, Western Blot, Control, Enzyme-linked Immunosorbent Assay

Journal: Annals of Translational Medicine

Article Title: NLRP3 mediates trophoblastic inflammasome activation and protects against Listeria monocytogenes infection during pregnancy

doi: 10.21037/atm-22-4120

Figure Lengend Snippet: Listeria monocytogenes induces inflammasome-mediated caspase-1 activation and IL-1β secretion in trophoblast cells. LPS-primed HTR-8/SVneo cells were stimulated with Listeria monocytogenes (MOI =500, 4 h). (A) Cell extracts were immunoblotted for IL-1β maturation. β-tubulin served as a loading control. (B) The culture supernatants were analyzed for IL-1β secretion. (C) Caspase-1 activity was determined as described in the “Methods” section. (D) HTR-8/SVneo cells were stimulated with Listeria monocytogenes (MOI =5, 4 h). Localization of NLRP3 (green) and nuclei (blue) using fluorescence microscopy (original magnification, × 400). The data are expressed as the means ± SE. ***, P<0.001, Listeria monocytogenes infection vs. the value for control group. IL, interleukin; LPS, lipopolysaccharide; MOI, multiplicity of infection; SE, standard deviation. n.s., not statistically significant; NLRP3, nucleotide-binding oligomerization domain-like receptor pyrin domain-containing 3.

Article Snippet: Rabbit anti-human cleaved IL-1β polyclonal antibody (#2021) was obtained from Cell Signaling Technology (CST, Shanghai, China). β-Tubulin monoclonal antibody (Tianjin Sungene Biotech, China) was used as an internal control.

Techniques: Activation Assay, Control, Activity Assay, Fluorescence, Microscopy, Infection, Standard Deviation, Binding Assay

Journal: Annals of Translational Medicine

Article Title: NLRP3 mediates trophoblastic inflammasome activation and protects against Listeria monocytogenes infection during pregnancy

doi: 10.21037/atm-22-4120

Figure Lengend Snippet: NLRP3 deficiency prevents Listeria monocytogenes -mediated IL-1β secretion in trophoblast cells. Primary mouse trophoblast cells from WT and NLRP3 −/− mice were isolated as described in the “Methods” section. IL-1β secretion in trophoblast cells infected with Listeria monocytogenes for indicated times. The data are expressed as the mean ± SE of three independent experiments. **, P<0.01. WT, wild-type; IL, interleukin; SE, standard deviation; NLRP3, nucleotide-binding oligomerization domain-like receptor pyrin domain-containing 3.

Article Snippet: Rabbit anti-human cleaved IL-1β polyclonal antibody (#2021) was obtained from Cell Signaling Technology (CST, Shanghai, China). β-Tubulin monoclonal antibody (Tianjin Sungene Biotech, China) was used as an internal control.

Techniques: Isolation, Infection, Standard Deviation, Binding Assay

Journal: Cell Death Discovery

Article Title: TLR2/caspase-5/Panx1 pathway mediates necrosis-induced NLRP3 inflammasome activation in macrophages during acute kidney injury

doi: 10.1038/s41420-022-01032-2

Figure Lengend Snippet: C57/BL mice were administered by intraperitoneal injection of cisplatin (25 mg/kg) and sacrificed 3 days later. Representative PAS staining images and immunohistochemical images F4/80 staining. Asterisk indicates injured renal tubules and white arrow indicates infiltrated macrophages. (scale bar, 100 μm) ( A ). Tubular injury score assessed in each group ( B ). Quantification of F4/80-positive cells by digital image analysis ( C ). Relative mRNA expressions of KIM-1 and NGAL in the renal tissue ( D ). Relative mRNA expressions of IL-1β in the renal tissue ( E ). Data are presented as the mean ± SD. n = 6 mice per group. The p values were calculated using Students t test. *** p < 0.001.

Article Snippet: Immunoblotting was performed using specific antibodies overnight at 4 °C using the following antibodies: primary antibodies rabbit polyclonal anti-human IL-1β (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-human caspase-1 (1:200, Santa Cruz Biotechnology), rabbit polyclonal anti-human Pannexin-1 (1:500, GeneTex, CA, USA), rabbit polyclonal anti-human P2RX7 (1:200, GeneTex), rabbit polyclonal anti-TLR2 (1:500, Abcam, Cambridge, USA), Mouse monoclonal anti-TLR4 (1:500, Abcam), anti-caspase-5 (1:500, Cell Signaling Technology).

Techniques: Injection, Staining, Immunohistochemical staining

Journal: Cell Death Discovery

Article Title: TLR2/caspase-5/Panx1 pathway mediates necrosis-induced NLRP3 inflammasome activation in macrophages during acute kidney injury

doi: 10.1038/s41420-022-01032-2

Figure Lengend Snippet: PMA-differentiated THP-1 cells were treated with or without 100 ng/ml LPS for 3 h before incubated with different concentrations of conditioned medium (CM, primary concentration; CM 1:1, 1:1 dilution with culture medium; CM 1:2, 1:2 dilution with culture medium) for 6 h, incubated with 5 mM ATP for 1 h (positive control) or incubated with medium from normal NRK-52E cells (Med) as control. Culture supernatants (Sup) and cell lysates (Lys) were collected. Representative immunoblot analyses of the immature (Pro-) and mature forms of caspase-1 and IL-1β in culture supernatants and cell lysates ( A ). Quantification of IL-1β in supernatant under different treatments by ELISA ( B ). Relative mRNA expressions of NLRP3, AIM2, NLRP1 and NLRC4 in THP-1 cells ( C ). Representative immunofluorescence images of ASC in THP-1 cells. Arrows: ASC specks (scale bar, 20 μm) ( D ). Representative immunoblot analysis of the immature (Pro-) and mature forms of caspase-1 and IL-1β in culture supernatants and cell lysates from THP-1 cells, Def-NLRP3-THP-1 cells, and Def-ASC-THP-1 cells ( E ). Quantification of IL-1β in supernatant of THP-1 cells under different treatments by ELISA ( F ). Data are presented as the mean ± SD of the representative experiment performed in at least three biological replicates. The p values were calculated using one-way ANOVA test. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Immunoblotting was performed using specific antibodies overnight at 4 °C using the following antibodies: primary antibodies rabbit polyclonal anti-human IL-1β (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-human caspase-1 (1:200, Santa Cruz Biotechnology), rabbit polyclonal anti-human Pannexin-1 (1:500, GeneTex, CA, USA), rabbit polyclonal anti-human P2RX7 (1:200, GeneTex), rabbit polyclonal anti-TLR2 (1:500, Abcam, Cambridge, USA), Mouse monoclonal anti-TLR4 (1:500, Abcam), anti-caspase-5 (1:500, Cell Signaling Technology).

Techniques: Incubation, Concentration Assay, Positive Control, Control, Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence

Journal: Cell Death Discovery

Article Title: TLR2/caspase-5/Panx1 pathway mediates necrosis-induced NLRP3 inflammasome activation in macrophages during acute kidney injury

doi: 10.1038/s41420-022-01032-2

Figure Lengend Snippet: Representative immunofluorescence images of ATP in PMA-differentiated THP-1 cells (scale bar, 20 μm) ( A ). Quantification of ATP level in culture supernatant and in cell lysate of THP-1 cells by ATP assay kit ( B , C ). Relative mRNA expressions of Panx1, P2RX4, and P2RX7 in THP-1 cells ( D ). Representative and quantitative immunoblot analyses of Panx1 and P2RX7 ( E , F ). PMA-differentiated THP-1 cells were pretreated with probenecid (100 μM, 200 μM) or suramin (100 μM, and 200 μM) for 1 h and further stimulated with CM for 6 h. Representative and quantitative immunoblot analyses of the immature and mature forms of IL-1β in culture supernatants and cell lysates of THP-1 cells treated with probenecid or suramin ( G , I ). Quantification of IL-1β in supernatant of THP-1 cells treated with probenecid or suramin by ELISA ( H , J ). Data are presented as the mean ± SD of the representative experiment performed in at least three biological replicates. The p values were calculated using Students t test or one-way ANOVA test. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Immunoblotting was performed using specific antibodies overnight at 4 °C using the following antibodies: primary antibodies rabbit polyclonal anti-human IL-1β (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-human caspase-1 (1:200, Santa Cruz Biotechnology), rabbit polyclonal anti-human Pannexin-1 (1:500, GeneTex, CA, USA), rabbit polyclonal anti-human P2RX7 (1:200, GeneTex), rabbit polyclonal anti-TLR2 (1:500, Abcam, Cambridge, USA), Mouse monoclonal anti-TLR4 (1:500, Abcam), anti-caspase-5 (1:500, Cell Signaling Technology).

Techniques: Immunofluorescence, ATP Assay, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Cell Death Discovery

Article Title: TLR2/caspase-5/Panx1 pathway mediates necrosis-induced NLRP3 inflammasome activation in macrophages during acute kidney injury

doi: 10.1038/s41420-022-01032-2

Figure Lengend Snippet: Relative mRNA expressions of TLR1, TLR2, TLR4, and TLR6 in THP-1 cells ( A ). Representative and quantitative immunoblot analysis of TLR2 in THP-1 cells ( B , C ). PMA-differentiated THP-1 cells were transfected with TLR2 siRNA for 36 h before incubated with CM. Representative and quantitative immunoblot analysis of the immature and mature forms of IL-1β in supernatant and cell lysates of THP-1 cells ( D ). Representative immunostaining images of ATP in THP-1 cells (scale bar, 30 μm) ( E ). Quantification of ATP in culture supernatant of THP-1 cells by ATP assay ( F ). Representative and quantitative immunoblot analyses of Panx1 and cleaved Panx1 in cell lysates of THP-1 cells ( G ). NC Negative control siRNA. Data are presented as the mean ± SD of the representative experiment performed in at least three biological replicates. The p values were calculated using Students t test or one-way ANOVA test. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Immunoblotting was performed using specific antibodies overnight at 4 °C using the following antibodies: primary antibodies rabbit polyclonal anti-human IL-1β (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-human caspase-1 (1:200, Santa Cruz Biotechnology), rabbit polyclonal anti-human Pannexin-1 (1:500, GeneTex, CA, USA), rabbit polyclonal anti-human P2RX7 (1:200, GeneTex), rabbit polyclonal anti-TLR2 (1:500, Abcam, Cambridge, USA), Mouse monoclonal anti-TLR4 (1:500, Abcam), anti-caspase-5 (1:500, Cell Signaling Technology).

Techniques: Western Blot, Transfection, Incubation, Immunostaining, ATP Assay, Negative Control

Journal: Cell Death Discovery

Article Title: TLR2/caspase-5/Panx1 pathway mediates necrosis-induced NLRP3 inflammasome activation in macrophages during acute kidney injury

doi: 10.1038/s41420-022-01032-2

Figure Lengend Snippet: Relative mRNA expressions of caspase-1, caspase-4, and caspase-5 in THP-1 cells ( A ). Representative and quantitative immunoblot analysis of caspase-5 in cell lysates of THP-1 cells ( B ). PMA-differentiated THP-1 cells were transfected with caspase-5 siRNA for 36 h before incubated with Med or CM. Quantification of ATP in culture supernatant of THP-1 cells by ATP assay ( C ). Representative immunoblot analyses of the immature and mature forms of IL-1β, Panx1 and cleaved Panx1, and caspase-5 in supernatant and cell lysates of THP-1 cells ( D ). Computational prediction of caspase-5 protein partners network using STRING database ( https://string-db.org ) ( E ). PMA-differentiated THP-1 cells were transfected with TLR2 siRNA for 36 h before incubated with Med or CM. Representative immunoblot analyses of the immature and mature forms of caspase-5 in cell lysates of THP-1 cells ( F ). NC Negative control siRNA. Data are presented as the mean ± SD of the representative experiment performed in at least three biological replicates. The p values were calculated using Students t test. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Immunoblotting was performed using specific antibodies overnight at 4 °C using the following antibodies: primary antibodies rabbit polyclonal anti-human IL-1β (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-human caspase-1 (1:200, Santa Cruz Biotechnology), rabbit polyclonal anti-human Pannexin-1 (1:500, GeneTex, CA, USA), rabbit polyclonal anti-human P2RX7 (1:200, GeneTex), rabbit polyclonal anti-TLR2 (1:500, Abcam, Cambridge, USA), Mouse monoclonal anti-TLR4 (1:500, Abcam), anti-caspase-5 (1:500, Cell Signaling Technology).

Techniques: Western Blot, Transfection, Incubation, ATP Assay, Negative Control

Journal: Cell Death Discovery

Article Title: TLR2/caspase-5/Panx1 pathway mediates necrosis-induced NLRP3 inflammasome activation in macrophages during acute kidney injury

doi: 10.1038/s41420-022-01032-2

Figure Lengend Snippet: NRK-52E cells were pre-incubated with 50 mM NAC before being treated with cisplatin and conditioned medium was collected (NAC-CM). Combined Annexin-V and 7-AAD staining was used to distinguish primary apoptotic (Annexin-V+/7-AAD−), primary necrotic (Annexin-V−/7-AAD+), secondary necrotic (Annexin-V+/7-AAD+), and live (Annexin-V−/7-AAD−) cells. Representative flow cytometry analysis and summarized results of TECs ( A , B ). The viability of TECs was analyzed by MTT assay ( C ). PMA-differentiated THP-1 cells were incubated with Med or CM or NAC-CM. Quantification of ATP in culture supernatant of THP-1 cells by ATP assay ( D ). Representative immunostaining images of ATP in THP-1 cells (scale bar, 30 μm) ( E ). Representative immunoblot analyses of the immature and mature forms of IL-1β, TLR2, Panx1, and cleaved Panx1 in supernatant and cell lysates of THP-1 cells ( F ). Data are presented as the mean ± SD of the representative experiment performed in at least three biological replicates. The p values were calculated using one-way ANOVA test. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Immunoblotting was performed using specific antibodies overnight at 4 °C using the following antibodies: primary antibodies rabbit polyclonal anti-human IL-1β (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-human caspase-1 (1:200, Santa Cruz Biotechnology), rabbit polyclonal anti-human Pannexin-1 (1:500, GeneTex, CA, USA), rabbit polyclonal anti-human P2RX7 (1:200, GeneTex), rabbit polyclonal anti-TLR2 (1:500, Abcam, Cambridge, USA), Mouse monoclonal anti-TLR4 (1:500, Abcam), anti-caspase-5 (1:500, Cell Signaling Technology).

Techniques: Incubation, Staining, Flow Cytometry, MTT Assay, ATP Assay, Immunostaining, Western Blot

Journal: Cell Death Discovery

Article Title: TLR2/caspase-5/Panx1 pathway mediates necrosis-induced NLRP3 inflammasome activation in macrophages during acute kidney injury

doi: 10.1038/s41420-022-01032-2

Figure Lengend Snippet: NAC (500 mg/kg) was given by oral gavage 3 days before cisplatin treatment and 24 h after cisplatin treatment. Serum BUN and creatinine were quantified 3 days after cisplatin treatment ( A , B ). Representative PAS staining images and immunohistochemical images F4/80 staining. Asterisk indicates injured renal tubules and white arrow indicates infiltrated macrophages. (scale bar, 100 μm) ( C ). Tubular injury score and quantification of F4/80-positive cells in each group ( D , E ). Relative mRNA expressions of IL-1β, NLRP3, Panx1, and TLR2 in renal tissue ( F – I ). CON, normal control. Data are presented as the mean ± SD. n = 6 at each group The p values were calculated using one-way ANOVA test. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Immunoblotting was performed using specific antibodies overnight at 4 °C using the following antibodies: primary antibodies rabbit polyclonal anti-human IL-1β (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-human caspase-1 (1:200, Santa Cruz Biotechnology), rabbit polyclonal anti-human Pannexin-1 (1:500, GeneTex, CA, USA), rabbit polyclonal anti-human P2RX7 (1:200, GeneTex), rabbit polyclonal anti-TLR2 (1:500, Abcam, Cambridge, USA), Mouse monoclonal anti-TLR4 (1:500, Abcam), anti-caspase-5 (1:500, Cell Signaling Technology).

Techniques: Staining, Immunohistochemical staining, Control

Journal: Cell Death Discovery

Article Title: TLR2/caspase-5/Panx1 pathway mediates necrosis-induced NLRP3 inflammasome activation in macrophages during acute kidney injury

doi: 10.1038/s41420-022-01032-2

Figure Lengend Snippet: We propose that danger signals from necrotic TECs can bind with TLR2 in macrophages, which upregulates caspase-5 and trigger Panx1 cleavage. Intracellular ATP released from macrophages through Panx1 channel can stimulate P2RX7 and cause the activation of NLRP3 inflammasome to produce IL-1β.

Article Snippet: Immunoblotting was performed using specific antibodies overnight at 4 °C using the following antibodies: primary antibodies rabbit polyclonal anti-human IL-1β (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-human caspase-1 (1:200, Santa Cruz Biotechnology), rabbit polyclonal anti-human Pannexin-1 (1:500, GeneTex, CA, USA), rabbit polyclonal anti-human P2RX7 (1:200, GeneTex), rabbit polyclonal anti-TLR2 (1:500, Abcam, Cambridge, USA), Mouse monoclonal anti-TLR4 (1:500, Abcam), anti-caspase-5 (1:500, Cell Signaling Technology).

Techniques: Activation Assay

Journal: Cell Death Discovery

Article Title: TLR2/caspase-5/Panx1 pathway mediates necrosis-induced NLRP3 inflammasome activation in macrophages during acute kidney injury

doi: 10.1038/s41420-022-01032-2

Figure Lengend Snippet: Primers used for real-time PCR.

Article Snippet: Immunoblotting was performed using specific antibodies overnight at 4 °C using the following antibodies: primary antibodies rabbit polyclonal anti-human IL-1β (1:200, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal anti-human caspase-1 (1:200, Santa Cruz Biotechnology), rabbit polyclonal anti-human Pannexin-1 (1:500, GeneTex, CA, USA), rabbit polyclonal anti-human P2RX7 (1:200, GeneTex), rabbit polyclonal anti-TLR2 (1:500, Abcam, Cambridge, USA), Mouse monoclonal anti-TLR4 (1:500, Abcam), anti-caspase-5 (1:500, Cell Signaling Technology).

Techniques:

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Detection of proteins associated with the pyroptosis signaling pathway in breast cancer tissues and their significance

doi:

Figure Lengend Snippet: Correlation of caspase1, IL-1β, and GSDMD expressions with clinicopathologic characteristics of patients with breast cancer

Article Snippet: Rabbit anti-human caspase-1 polyclonal antibody, rabbit anti-human IL-1β polyclonal antibody and rabbit anti-human GSDMD polyclonal antibody were purchased from Proteintech, USA; ElivisionTM plus kit and DAB color development kit were purchased from Fuzhou Maixin Biotechnology.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Detection of proteins associated with the pyroptosis signaling pathway in breast cancer tissues and their significance

doi:

Figure Lengend Snippet: Expression of caspase1, IL-1β, and GSDMD protein in breast cancers (Elivision, original magnification: ×100). A: Caspase1 is highly expressed in breast cancer tissues; B: Low expression of caspase1 in breast cancer; C: IL-1β is highly expressed in breast cancer tissues; D: Low expression of IL-1β in breast cancer; E: GSDMD is highly expressed in breast cancer tissues; F: Low expression of GSDMD in breast cancer.

Article Snippet: Rabbit anti-human caspase-1 polyclonal antibody, rabbit anti-human IL-1β polyclonal antibody and rabbit anti-human GSDMD polyclonal antibody were purchased from Proteintech, USA; ElivisionTM plus kit and DAB color development kit were purchased from Fuzhou Maixin Biotechnology.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Detection of proteins associated with the pyroptosis signaling pathway in breast cancer tissues and their significance

doi:

Figure Lengend Snippet: Relationship of caspase1, IL-1β, and GSDMD expressions in breast cancer

Article Snippet: Rabbit anti-human caspase-1 polyclonal antibody, rabbit anti-human IL-1β polyclonal antibody and rabbit anti-human GSDMD polyclonal antibody were purchased from Proteintech, USA; ElivisionTM plus kit and DAB color development kit were purchased from Fuzhou Maixin Biotechnology.

Techniques: Expressing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Detection of proteins associated with the pyroptosis signaling pathway in breast cancer tissues and their significance

doi:

Figure Lengend Snippet: Multivariate survival analysis of 108 patients with breast cancer

Article Snippet: Rabbit anti-human caspase-1 polyclonal antibody, rabbit anti-human IL-1β polyclonal antibody and rabbit anti-human GSDMD polyclonal antibody were purchased from Proteintech, USA; ElivisionTM plus kit and DAB color development kit were purchased from Fuzhou Maixin Biotechnology.

Techniques:

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Detection of proteins associated with the pyroptosis signaling pathway in breast cancer tissues and their significance

doi:

Figure Lengend Snippet: Survival curves of breast cancer patients with caspase1 (A), IL-1β (B) and GSDMD (C) protein high expression group and low expression group.

Article Snippet: Rabbit anti-human caspase-1 polyclonal antibody, rabbit anti-human IL-1β polyclonal antibody and rabbit anti-human GSDMD polyclonal antibody were purchased from Proteintech, USA; ElivisionTM plus kit and DAB color development kit were purchased from Fuzhou Maixin Biotechnology.

Techniques: Expressing